Scientific manager: Béatrice Turcq
Technical manager: Valérie Prouzet-Mauleon
CRISP’edit
You want to use CRISPR-Cas9 technology to:
- Genetic KO
- Modify a gene sequence
- Perform functional screening (CRISPR libraries)
Presentation
The CRISP’edit platform helps you with the design or makes your modifications.
Several points will be evaluated for a successful use of CRISPR technology: the vectorization strategy inside the cells, the best technique according to the project (double-stranded or single-stranded cut of DNA, base-editing, prime-editing) , the best screening strategy for modified cells, the different stages of a functional screening project (CRISPR banks).
Services
CRISP’edit services
Our services and consulting support cover the design, implementation and analysis of CRISPR experiments.
Design and realization of KO / KI / Punctual Mutations experiments
Design and execution of experiments using sgRNA libraries, screen selection, sgRNA sequencing, bioinformatics analysis of results
Choosing the appropriate CRISPR system
sgRNA design, recombination templates
Vectorization strategy according to the cell model
Efficacy Screening Strategy
Rates: quote on request
Technology
CRISPR-Cas9 technology enables targeted DNA cutting in living cells. This cut will be repaired with the appearance of errors (Indels) or by using a recombination matrix. This system is very powerful when the targeting of Cas9 is effective thanks to suitably designed gRNAs and when the vectorization means (plasmid DNA, ribonucleoprotein, lentivirus) is adapted to the project and the cell type.
project design
characterization of clones
Contact
Scientific manager
Béatrice Turcq
05.57.57.10.51
Technical manager
Valérie Prouzet-Mauleon
valerie.prouzet-mauleon@u-bordeaux.fr
05.57.57.45.47
Research engineer
Christelle Saint-Marc
Christelle.Saint-Marc@u-bordeaux.fr
05.57.57.45.47
Assistant engineer
Noé Calais-Yager
noe.calais-yager@u-bordeaux.fr
05.57.57.45.47
Location
CRISP’edit – UAR TBMCore
BBS Building, 4th floor
Office N4029
2 rue Dr Hoffmann Martinot
33000 Bordeaux
Our team
Béatrice
Turcq
Valérie
Prouzet-Mauleon
Christelle
Saint-Marc
