Scientific and technical manager:
Laetitia Andrique
VoxCell
You want to:
- Establish a 3D cell culture project
- Mimic the tumour complexity
- Proceed to drug screening on organoids
- Investigate the role of mechanics in a cell population organised in a 3D fashion
VoxCell services
The VoxCell interdisciplinary facility combines a Microfluidics technique with Biology to generate organoids and spheroids, or more generally 3D cell mono- or co-cultures.
Help for the design of your project
Cell encapsulation (in capsules or tubes)
Sample preparation
Quality control
Provision of small equipment for the analysis
Help and training for the manipulation of the spheroids/3D objects
Prices : on demand
Technology
Cell-capsule technology
The ‘Cellular Capsule Technology’ working principle starts with co-extruding a cell suspension and a polymer solution. By contacting a calcium bath, the polymer solution undergoes gelation resulting in cell-laden spherical and hollow capsules . The capsule is permeable to nutrients and favours the 3D assembly and self-organisation of the tissue or tumour microenvironment. Capsules are produced in a high throughput format (up to hundreds of thousands per encapsulation).
Technology benefits…
1. Cell self-organization
– Cell capsules: e.g. HEK 293T tumor spheroids
2. High throughput
- Around 5000 capsules/second
- 1 to 2 meters of tubes in 10 minutes
3. Drug testing
4. Mechanical forces
Equipment
Available equipment
- Culture room
- Encapsulation set-up
- 3D printers
- Analyse equipment
- Pump for perfusing 3D objects directly into an incubator
- Incubascope: high-throughput 3D object imaging system, placed in a dedicated incubator.
(https://pubmed.ncbi.nlm.nih.gov/35154792/)
Contact
Information and location
Scientific & Technical Authority
Laetitia Andrique
laetitia.andrique@u-bordeaux.fr
06.63.21.33.50
Scientific Support
Pierre Nassoy – Microfluidics
pierre.nassoy@u-bordeaux.fr
05.57.01.72.09
Gaëlle Recher – 3D imaging
gaelle.recher@institutoptique.fr
05.57.01.72.69
Audrey Ferrand – Organoids Thematic Network, Inserm
audrey.ferrand@inserm.fr
Staff and students
Vanessa Bergeron, research engineer
Specialized in encapsulation, tissue engineering and 3D-object analysis
vanessa.bergeron@u-bordeaux.fr
Aurélien Richard, PhD student 2022-2025
Development of new 3D mammary cell models
aurelien.richard@u-bordeaux.fr
R&D staff
Amaury Badon, CRCN CNRS, LP2N Biof team
Spécialiste en optique et développement de nouvelles technologies pour la microscopie 3D
amaury.badon@institutoptique.fr
Adeline Boyreau, Ingénieure d’étude R&D, LP2N équipe Biof
Soutien R&D dans la transparisation et imagerie 3D
adeline.boyreau@u-bordeaux.fr
Location
TBMCore
INSERM US 005 – CNRS UAR 3427
University of Bordeaux
BBS building
2 rue Dr Hoffmann Martinot
33076 Bordeaux
VoxCell
Our team
Laetitia
Andrique
Vanessa
Bergeron
Aurélien
Richard
Pierre
Nassoy