Scientific manager: Jean-Max Pasquet
Technical manager: Arnaud Villacreces
CellOxia
You want to:
- Perform in vitro culture experiments under real tissue conditions,
- Use the services of the platform engineer to carry out an in vitro hypoxia study,
- To be advised in the design of a cell culture experiment in hypoxia.
Overview
The CellOxia platform
While more than 99% of labs grow cells in a gaseous atmosphere of ~21% O2, O2 concentrations within body tissues are much lower (Figure 1). Oxygen is fundamental in cellular physiology and the mechanisms by which cells adapt to oxygen levels have been highlighted thanks to the work of the three winners of the 2019 Nobel Prize in Physiology or Medicine.
Figure 1: Partial pressure of O2 in tissues (Mas-Bargues, Int. J. Mol. Sci. 2019)
The CellOxia platform offers public and private laboratories equipment (XVivo Biosphérix) for carrying out experiments and/or incubating cells under controlled atmospheric conditions (from 0.1 to 10% O2; from 0 to 15% CO2; 37°C). It is possible to reproduce in vitro physiological or physiopathological parameters existing within a tissue. Thanks to its “Paula” (Leica) imager, users will be able to observe their crops without any disruption of atmospheric conditions.
Services
CellOxia services
The CellOxia platform gives you access to equipment and skilled personnel that will enable you to carry out cell culture experiments in hypoxia.
Access to equipment after prior training.
Tips for designing cell culture experiments in hypoxia.
Engineering services for your in vitro hypoxia studies.
Equipment
1 – XVivo X1 Biospherix model
- 3 independent controlled-atmosphere incubators: O2, CO2, temperature
- 1 controlled-atmosphere workspace: O2, CO2, temperature
- 1 transfer chamber
2 – Equipment in the P2 culture laboratory
- 1 120 cm Class II microbiological safety cabinet
- 1 normoxic incubator
- 1 centrifuge
- Consumables, etc.
3 – PAULA Leica smart cell imaging system
- x10 objective with x3 digital zoom
- 2 fluorescent LEDs: red and green
- Time-lapse, wound-healing assay, transfection efficiency, etc.
Organization
Schedule, booking and user charter
Schedule and booking
The platform is freely accessible according to its availability for trained and autonomous users.
For other users, technical assistance is available by appointment. In any case, the devices must be reserved at least 72 hours in advance.
User charter
Any user of the platform must have read and signed the user charter in order to access the service.
Contact
Scientific officer
Jean-Max Pasquet
jean-max.pasquet@u-bordeaux.fr
07.85.42.59.25
Technical manager
Arnaud Villacreces
arnaud.villacreces@u-bordeaux.fr
05.57.57.16.11
Location
UAR TBMCore 3427 – US005
BBS Building – 4th floor – Office N.4032
University of Bordeaux, Carreire campus
2 rue du Docteur Hoffmann Martinot
33000 Bordeaux
Our team
Jean-Max
Pasquet
