Scientific lead: Julie Dechanet-Merville
Flow cytometry with cell sorter
Flow cytometry
- Analyze and phenotype your biological samples
- Sort your cell and particle suspensions
- Get advice for setting up your antibody panels and your markings
Bordeaux Cytometry
FACSility platform services
FACSility offers a range of instruments for the analysis of 16 fluorescences
Equipment after training
Feasibility study
Scientific cooperation
Rates: Invoicing of time consumed
Specificities: Sorter under PSM, wide choice of analyzers and optical configurations, spectral cytometry
Quality: Online instrument reservation, data storage server, 4 Diva / Flowjo analysis stations, Spectroflo, 24-hour service
What is cytometry?
It makes it possible to measure on a suspension of particles (cells, bacteria, parasites, beads, etc.) the individual characteristics of each particle such as size, shape and complexity and any component or function that can be detected by a fluorescent compound.
On the Thermo Fisher Scientific website, you will find two excellent presentations in English: introduction to cytometry et result analysis.
The excitation and emission spectra of the main fluorochromes are available through the following links: BD fluorescence spectrum ou Molecular Probes fluorescence spectrum.
Further information on cytometry is available from the International Society of Analytical Cytology.
Training
The platform offers two practical 2.5-day training courses in multicolor analysis and cell sorting. These trainings are qualifying and labeled CNRS enterprise.
Hands-on multicolor analysis training
Flow cytometry.
Equipment
Analyzers and sorters
Analyzers
- One 28-color Symphony A3
- Two 8-color Canto II instruments with plate loaders
- One 16-color Fortessa with plate loader
- One Aurora Spectral, 67 parameters, 5 lasers (UV, V, B, YG, R)
Sorters
- One 9-color Melody
- One Aurora Spectral, 40 parameters, 4 lasers (V, B, YG, R), 6-way sorting
Equipment details
BD FACSymphony™ A3
The BD FACSymphony A3 offers 30 parameters across up to five lasers, enabling the use of new dyes with improved brightness and spillover characteristics. The ability to develop panels for broader phenotyping, deeper interrogation of cell subsets and improved panels with higher resolution enables researchers to gain deeper scientific insight.
Further technical information is available on the BD FACSymphony A3.
BD FACSCanto II Analyzer
The BD FACSCanto II analyzer is equipped with three lasers at 488 nm, 633 nm and 407 nm. It enables the simultaneous analysis of 8 fluorescence channels. It is equipped with a high-throughput sampler, allowing automated processing of plate-based samples.
More technical information is available on the BD FACSCanto II.
BD LSRFortessa Analyzer
The BD LSRFortessa analyzer is equipped with four lasers at 405 nm, 488 nm, 561 nm and 640 nm. The 560 nm laser enables optimal excitation of red fluorochromes (phycoerythrin, DsRed, Tomato, Cherry, etc.). This instrument enables simultaneous analysis of 16 fluorescence channels and is equipped with a high-throughput plate loader.
More technical information is available on the BD LSRFortessa.
Cytek Aurora Analyzer
The Aurora spectral analyzer is equipped with 5 lasers: UV at 355 nm, Violet at 405 nm, Blue at 488 nm, Yellow / Green at 561 nm and Red at 640 nm. It has 64 fluorescence detectors and 3 morphology detectors. Measuring spectral signature differences across all lasers and detectors makes it possible to combine fluorochromes that are incompatible in conventional cytometry.
The OMIP-069 was published in October 2020 with the analysis of 40 markers in human blood.
More technical information is available on the Cytek Aurora.
Cytek Aurora CS Sorter
The Aurora CS spectral sorter is equipped with 4 lasers: Violet at 405 nm, Blue at 488 nm, Yellow / Green at 561 nm and Red at 640 nm. It has 40 fluorescence detectors and 3 morphology detectors. Like the Aurora analyzer, the CS offers the benefits of spectral analysis technology. Its optical design and unmixing algorithm provide greater flexibility, enabling the use of a wide range of new fluorochrome combinations. With sorting of up to 6 ways, nozzle choices and customizable sorting modes, automated setup and sort-stream monitoring, the CS provides the flexibility needed for all applications.
More technical information is available on the Cytek Aurora CS.
BD FACSMelody Sorter
The BD FACSMelody™ is a single-nozzle 100 µm sorter compatible with all cell types, enabling up to 4 populations to be isolated simultaneously in tubes or plates. The 3-laser, 9-fluorescence-parameter optical configuration (3V, 4B, 2R) makes it easy to identify most of the cell populations you need to sort. Automated startup and sort setup, combined with BD FACSChorus software, make it possible to carry out your sorting runs independently.
More technical information is available on the BD FACSMelody.
Available software
Contact
Contact us
Email : tbmcore-cytometrie@u-bordeaux.fr
Phone: 05.57.57.57.05
University of Bordeaux
FACSility Platform
UMS 3427 / US 005 Laboratory
BBS Building, 5th floor, S.5085
2 rue Hoffmann Martinot
33076 BORDEAUX Cedex
Data storage
The use of USB drives or external hard drives is strictly prohibited. Use the server share when you are in the cytometry facility, or the “Mobilité” VPN when you are outside the service, to access your backup space on the laboratory server.
Internal rules and project submission
Every user of the Shared Cytometry Service must have read the internal rules and completed the online form in the last paragraph of the document in order to access the service.
Every project request must be submitted by the project lead to the steering committee using the online form.
Schedule and booking
The flow cytometers are freely accessible, subject to availability, for trained and independent users. Technical assistance is available by appointment for other users.
Sorting runs on the Aria and Aurora CS are performed only by platform staff, who must be notified no later than the Friday of the previous week. Mycoplasma-contaminated cells or samples presenting a biological hazard above level 2 (BSL-2/P2) will be refused.
In all cases, instruments must be booked at least 24 hours in advance using the following link:
Our team
JULIE
DÉCHANET-MERVILLE
ATIKA
ZOUINE
VINCENT
PITARD
